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Enzymatic hydrolysis could be executed utilizing either a single enzyme or a combination of numerous enzymes as being a pre-treatment to liberate bioactive compounds more efficiently from purely natural by-products and solutions [eleven]. Lots of scientific studies have shown that using proteases like Alcalase, pepsin, and trypsin to hydrolyze delipidated and freeze-dried fish roe can generate peptides with antioxidant exercise, antibacterial Houses, anticancer Homes and enzyme inhibitory consequences [2,12]. While in the existing research, a different commercial bacterial 23hoki slot protease, HT, plus a fungal protease, FP-II had been investigated for his or her abilities to supply antioxidant fish roe hydrolysates from Gemfish and Hoki roes and Alcalase was applied together with for a reference enzyme for comparative needs.

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